Human Oral Microbiome Taxon Description
Treponema lecithinolyticum
Human Oral Taxon ID (HOT):653Synonym:
Treponema sp. IV:17C:GF6
Status:
Named - Cultured
Type Strain:
ATCC 700332, OMZ 684
More info at StrainInfo
Classification:
Domain:Bacteria
Phylum:Spirochaetes
Class:Spirochaetia
Order:Spirochaetales
Family:Spirochaetaceae
Genus:Treponema
Species:lecithinolyticum
NCBI Taxonomy ID:
16S rRNA Sequence:
AF023040  [Entrez Link]
PubMed Search:15  [PubMed Link]
16S rRNA Alignment: View Alignment         Download Alignment         NoteNucleotide Search:114  [Entrez Nucleotide Link]
Phylogeny: View 16S rRNA tree         View all Tree filesProtein Search:4359  [Entrez Protein Link]
Prevalence by Molecular Cloning:
Clones seen = 13 / 34879 = 0.0373%
Rank Abundance = Tied for 244
Genome Sequence
2 View Genomes
 
Hierarchy Structure:    Hide or show the hierarchy structure
General Information:
Member of o­ne of the 10 phylogenetic groups of oral treponemes [1,4]. Originally detected in subjects with periodontitis as not-yet-cultivated phylotype (Group IV) termed Treponema sp. IV:17C:GF6 [4].  Closest cultivable relative is Treponema maltophilum, which also falls in Group IV.
Cultivability:
Growth o­n OMIZ-Pat-w/oPC agarose [1], are off-white, diffuse (~3 mm), subsurface colonies after 7 days incubation at 37oC.  Will not grow in chemically defined media, e.g., OMIZ-W1 [1], but requires yeast extract or Neopeptone
Phenotypic Characteristics:
Obligately anaerobic, catalase negative, helically coiled with translational motility in broth, especially in media of high viscosity [1].  Will creep o­n solid surfaces, such as agar media or glass.  Cells are Gram-negative,  0.15 um by 5 um, with a 1-2-1 periplasmic flagellar arrangement.

N-acetylglucosamine is required for growth, and D-arabinose, L-fucose or D-ribose enhance growth.  Other carbohydrates do not appear to support growth. Lecithin and 1% fetal calf serum inhibits growth.  Strains are resistant to rifampicin and fosfomycin.

High activities for alkaline phosphatase, acid phosphatase, B-galactosidase, B-glucuronidase, N-acetyl-B-glucosaminidase, phospholipase A, and phospholipase C
Prevalence and Source:

Detected in human subgingival plaque, most often in subjects with periodontitis

Disease Associations:
Association (e.g. diseased vs control sites) in patients with adult periodontitis and rapidly progressive periodontitis [1,3] and endodontic lesions [2].  Strong activities of phospholipase A and phospholipase C may serve as potential virulence factors [1]
References:
PubMed database:
[1] Wyss C, Choi BK, SchŘpbach P, Moter A, Guggenheim B, G÷bel UB. Treponema lecithinolyticum sp. nov., a small saccharolytic spirochaete with phospholipase A and C activities associated with periodontal diseases. Int J Syst Bacteriol.1999 Oct;49 Pt 4:1329-39  [PubMed]
[2] Siqueira JF Jr, R˘šas IN. PCR-based identification of Treponema maltophilum, T amylovorum, T medium, and T lecithinolyticum in primary root canal infections. Arch Oral Biol.2003 Jul;48(7):495-502  [PubMed]
[3] Moter A, Riep B, Haban V, Heuner K, Siebert G, Berning M, Wyss C, Ehmke B, Flemmig TF, G÷bel UB. Molecular epidemiology of oral treponemes in patients with periodontitis and in periodontitis-resistant subjects. J Clin Microbiol.2006 Sep;44(9):3078-85  [PubMed]
[4] Dewhirst FE, Tamer MA, Ericson RE, Lau CN, Levanos VA, Boches SK, Galvin JL, Paster BJ. The diversity of periodontal spirochetes by 16S rRNA analysis. Oral Microbiol Immunol.2000 Jun;15(3):196-202  [PubMed]
   
Curator:  
Creation Info:   Latest Modification:  wenhan,  2008-04-10 11:58:57

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