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Forsyth Bioinformatics

 
BLAST-Based Open-Reference 16S sRNA NGS
Species-Level Read Assignment Pipeline With QIIME Results
Code Category Minimal read count per species (MC)
(Click for detail report)
MC1 (All) MC10 MC100
A Total reads 7,261,424 7,261,424 7,261,424
B Total samples 119 119 119
C Samples with reads ≥ 500 reads (S≥500) 108 108 108
D Samples with reads < 500 reads (S<500) 11 11 11
E Reads in samples S≥500 7,261,179 7,261,179 7,261,179
F Reads in samples S<500 (removed) 245 245 245
G Total reads assigned 4,033,832 4,028,701 3,997,833
H Reads assigned to single species 1,267,262 1,266,349 1,260,969
I Reads assigned to multiple species 338,300 338,012 335,780
J Reads assigned to novel species 2,428,270 2,424,340 2,401,084
K Number of single species 580 275 115
L Number of multi-species 171 92 26
M Number of novel species 2,609 1,326 667
N Total reads unassigned 3,227,347 3,232,478 3,263,346
O Reads excluded (<MC) 0 5,131 35,999
P Chimeric reads 424,522 424,522 424,522
Q Reads without BLASTN hits 20,060 20,060 20,060
R Singleton reads 2,782,765 2,782,765 2,782,765
1.
A=E+F
A=F+G+N
G=H+I+J
N=O+P+Q+R
2.View all read count by samples
3.All data can be downloaded in a compressed ZIP file ZIP file
4.All data are also available on the FTP site for 30 days
5.Please read DOCUMENTATION (pdf)
6.Preferred web browser: Google Chrome or FireFox, especially for 2D and 3D plots
7.Contact: George Chen, Email: tchen\@forsyth.org for questions